April 23, 2003Nucleic Acids ResearchOpen Access

RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III

View Full Paper

Ask AI

Bookmark

Population

In vitro model using Escherichia coli ribonuclease III and RNA derived from T7 phage R1.1 RNase III substrate

Comparison

R1.1[CL3B] RNA vs R1.1 RNA (wild-type substrate)

Design

Preclinical

Authors

ICIrina Calin‐JagemanDominican University

Discussion

Member takes

Overview

Uncoupling of RNase III binding and cleavage by RNA motifs; leaves open in vivo roles and applications in bacterial RNA engineering.

Structured PICO

Population

In vitro model using Escherichia coli ribonuclease III and RNA derived from T7 phage R1.1 RNase III substrate

Intervention

R1.1[CL3B] RNA (mutated RNA with a bulge-helix-bulge motif)

Comparator

R1.1 RNA (wild-type substrate)

Outcome

Cleavage resistance and binding affinitysurrogate

The study provides direct evidence that E. coli RNase III can bind RNA without cleaving it, mediated by specific RNA structural motifs.

Cite This Study

Irina Calin‐Jageman (2003) studied this question.

synapsesocial.com/papers/6a70b85dfebe604dd7097c87 https://doi.org/10.1093/nar/gkg329

View Full Paper

Ask AI

Bookmark

April 23, 2003Nucleic Acids ResearchOpen Access

RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III

View Full Paper

Ask AI

Bookmark

Population

In vitro model using Escherichia coli ribonuclease III and RNA derived from T7 phage R1.1 RNase III substrate

Comparison

R1.1[CL3B] RNA vs R1.1 RNA (wild-type substrate)

Design

Preclinical

Authors

ICIrina Calin‐JagemanDominican University

Discussion

Member takes

Overview

Uncoupling of RNase III binding and cleavage by RNA motifs; leaves open in vivo roles and applications in bacterial RNA engineering.

Structured PICO

Population

In vitro model using Escherichia coli ribonuclease III and RNA derived from T7 phage R1.1 RNase III substrate

Intervention

R1.1[CL3B] RNA (mutated RNA with a bulge-helix-bulge motif)

Comparator

R1.1 RNA (wild-type substrate)

Outcome

Cleavage resistance and binding affinitysurrogate

The study provides direct evidence that E. coli RNase III can bind RNA without cleaving it, mediated by specific RNA structural motifs.

Cite This Study

Irina Calin‐Jageman (2003) studied this question.

synapsesocial.com/papers/6a70b85dfebe604dd7097c87 https://doi.org/10.1093/nar/gkg329

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark