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January 1, 1973Human Heredity

Superoxide Dismutase Isozymes in Different Human Tissues, Their Genetic Control and Intracellular Localization

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Authors

GBG. BeckmanUniversity of Hawaiʻi at MānoaELErik LundgrenUniversity Hospital of UmeåATArne TärnvikUmeå University

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Implication

Laboratory analysis reveals distinct subcellular localizations and separate genetic loci for superoxide dismutase isozymes in human tissues, indicating independent enzymatic regulation.

Key Points

  • To investigate the tissue distribution, intracellular localization, and genetic control of superoxide dismutase isozymes in human cells and organs.
  • Extracted superoxide dismutase isozymes from human organs, tissues, cell cultures, erythrocytes, and polymorphonuclear leucocytes.
  • Separated soluble and mitochondria-enriched cell fractions using differential centrifugation.
  • Analyzed electrophoretic phenotypes to evaluate genetic loci and subunit composition.
  • Isozyme A localized strictly to the soluble intracellular phase, whereas isozyme B concentrated primarily in mitochondria-enriched fractions.
  • Isozyme B was completely absent from erythrocytes and isozyme A was absent from polymorphonuclear leucocytes, while other human tissues expressed both isozymes in variable proportions.
  • Identified separate gene loci controlling each isozyme, with hybrid enzyme formation in heterozygous individuals demonstrating that isozyme A is a dimeric protein of two identical subunits.

Cite This Study

Beckman et al. (1973) studied this question.

synapsesocial.com/papers/6a70c1198031ec7bb1dc8cb6https://doi.org/10.1159/000152594
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