What question did this study set out to answer?

To determine whether fibroblast growth factor stimulates mitogenesis through polyphosphoinositide breakdown and protein kinase C activation in hamster fibroblasts.

December 1, 1986Journal of Biological ChemistryOpen Access

The mitogenic signaling pathway of fibroblast growth factor is not mediated through polyphosphoinositide hydrolysis and protein kinase C activation in hamster fibroblasts.

View Full Paper

Ask AI

Bookmark

Authors

IMI MagnaldoCentre National de la Recherche Scientifique GLGilles L’AllemainCentre National de la Recherche Scientifique JCJean‐Claude ChambardCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

In vitro study demonstrates fibroblast growth factor drives DNA synthesis without inositol lipid breakdown in hamster fibroblasts, indicating distinct mitogenic pathways.

Key Points

To determine whether fibroblast growth factor stimulates mitogenesis through polyphosphoinositide breakdown and protein kinase C activation in hamster fibroblasts.
Treated G0-arrested Chinese hamster lung fibroblasts (CCL39) with basic FGF (50 ng/ml), acidic FGF, or alpha-thrombin (1 unit/ml).
Assessed cytoplasmic alkalinization, intracellular Ca2+ levels via quin-2 fluorescence, S6 phosphorylation, c-myc expression, and DNA synthesis by nuclear labeling.
Measured inositol phosphate accumulation in the presence of 20 mM Li+ and evaluated protein kinase C activation using 125I-epidermal growth factor receptor transmodulation assays.
Basic FGF and alpha-thrombin both induced early mitogenic events, including cytoplasmic alkalinization (0.2-0.3 pH units) and a 30- to 40-fold increase in labeled nuclei.
FGF failed to generate detectable inositol bisphosphate or inositol trisphosphate, with total inositol phosphate levels reaching under 8% of the levels produced by thrombin.
FGF-mediated intracellular Ca2+ increases strictly required external Ca2+, and FGF did not induce protein kinase C-dependent inhibition of 125I-EGF binding, which thrombin reduced by 50-60%.

Cite This Study

Magnaldo et al. (1986) studied this question.

synapsesocial.com/papers/6a70c412e71d69abee08d402 https://doi.org/10.1016/s0021-9258(19)75976-6

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates fibroblast growth factor drives DNA synthesis without inositol lipid breakdown in hamster fibroblasts, indicating distinct mitogenic pathways.

The mitogenic signaling pathway of fibroblast growth factor is not mediated through polyphosphoinositide hydrolysis and protein kinase C activation in hamster fibroblasts.

Discussion

Member takes

Implication

Key Points

Cite This Study

The mitogenic signaling pathway of fibroblast growth factor is not mediated through polyphosphoinositide hydrolysis and protein kinase C activation in hamster fibroblasts.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider