Sex-specific regulation of IL-10 production in human adipose tissue in obesity

Figures

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  Figure 1. IL-10 secretion and mRNA expression in scWAT and serum of men and women. Three metabolic groups (non-obese healthy, non-obese T2D and obese T2D) of both women and men are represented by box plots. IL-10 secretion in WAT explants (A), circulating serum IL-10 (B) and IL10 mRNA expression in scWAT (C) are shown. Two-way ANOVA with Tukey’s and Sidak’s multiple comparisons were used in (A–C) to compare between the groups in men and women and to compare the groups between men and women, respectively. Patients from cohort 1 have been used for this analysis and number of individuals per group corresponds to Table 1 , *** <0.001, ** <0.01, * <0.05.

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  Figure 2. Amount of IL-10 secreting macrophages is increased in scWAT of women with obesity and T2D but not in men. (A) A flow cytometry gating strategy. Selection of alive single cells (four panels on the left), macrophage/monocyte (CD45+/CD14+) and M1 macrophage (CD45+/CD14+/CD206+/CD11c+) selection (two right-side panels). (B) The M1 macrophage population was then analyzed for IL-10 secretion. Representative contour plots from two individuals/group are shown for obese T2D and non-obese healthy women and men. FMO control samples are shown on the left side. (C) Compiled results of IL-10-secreting macrophage populations for non-obese healthy (n=8 for both men and women) and obese T2D (n=7 for women, n=8 for men) men and women from cohort 1. Two-way ANOVA with Tukey’s and Sidak’s multiple comparisons were used in (C) to compare between the groups in men and women and to compare the groups between men and women respectively. *** <0.001, * <0.05.

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  Figure 3. E1 has no effect on IL-10 expression neither in in vitro stimulated THP-1 macrophages nor in primary WAT SVF. (A) Relative IL10 mRNA expression in E1, TS, E2 and TNFα-treated THP-1 macrophages. Two-way ANOVA with Sidak’s multiple comparison was used to test the significance. ** <0.01 (n=4). (B) Relative IL10 mRNA in E1-stimulated WAT SVF; (Mann-whitney test, n=4 subjects in triplicates).