What question did this study set out to answer?

To determine whether serotonin-induced enhancement of transmitter release at the neuromuscular junction is driven by changes in resting intracellular calcium levels or action-potential-evoked calcium influx.

September 1, 1991Journal of NeuroscienceOpen Access

Presynaptic calcium and serotonin-mediated enhancement of transmitter release at crayfish neuromuscular junction

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Authors

KDKerry R. DelaneyUniversity of Victoria DTDW TankAT&T (United States)RZRS ZuckerUniversity of California, Berkeley

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Implication

Experimental study shows serotonin enhances transmitter release without altering presynaptic calcium dynamics in crayfish terminals, suggesting direct modulation of the neurosecretory apparatus.

Key Points

To determine whether serotonin-induced enhancement of transmitter release at the neuromuscular junction is driven by changes in resting intracellular calcium levels or action-potential-evoked calcium influx.
Conducted simultaneous Fura-2 microfluorometric calcium measurements and excitatory junction potential recordings in motor nerve terminals of crayfish (Procambarus clarkii) walking legs across 0.5–16 Hz stimulation.
Assessed presynaptic calcium and transmitter release before, during, and after 5-minute applications of 5–100 μM serotonin, including trials with presynaptic microinjection of the calcium buffer EGTA.
Serotonin enhanced excitatory junction potentials and spontaneous transmitter release without causing a detectable increase in resting intracellular calcium or calcium accumulation during action potential trains.
Intracellular injection of EGTA diminished frequency-dependent facilitation during high-frequency stimulation but did not reduce serotonin-mediated enhancement of excitatory junction potentials.

Cite This Study

Delaney et al. (1991) studied this question.

synapsesocial.com/papers/6a70c7bdf44fa9f079de7b9d https://doi.org/10.1523/jneurosci.11-09-02631.1991

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Implication

Experimental study shows serotonin enhances transmitter release without altering presynaptic calcium dynamics in crayfish terminals, suggesting direct modulation of the neurosecretory apparatus.

Presynaptic calcium and serotonin-mediated enhancement of transmitter release at crayfish neuromuscular junction

Discussion

Member takes

Implication

Key Points

Cite This Study

Presynaptic calcium and serotonin-mediated enhancement of transmitter release at crayfish neuromuscular junction

Discussion

Member takes

Implication

Key Points

Cite This Study

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