September 1, 2003Journal of Biological ChemistryOpen Access

Identification of a Functionally Critical Protein Kinase C Phosphorylation Residue of Cardiac Troponin T

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Population

Detergent-treated mouse left ventricular papillary muscle fiber bundles

Comparison

Recombinant troponin complex containing cardiac… vs Wild-type cardiac Troponin T (cTnT-wt)

Design

Preclinical

Authors

MSMarius P. SumandeaBioMarin (United States)WPW. Glen PyleDalhousie University TKTomoyoshi KobayashiIllinois College

Discussion

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Implication

Should not change clinical practice; hypothesis-generating for PKC regulation of troponin T in models.

Structured PICO

Population

Detergent-treated mouse left ventricular papillary muscle fiber bundles

Intervention

Recombinant troponin complex containing cardiac Troponin T (cTnT) mutated at PKC-dependent phosphorylation sites (Thr197, Ser201, Thr206, Thr287) to glutamate (E) or alanine (A), or phosphorylated by PKC-alpha

Comparator

Wild-type cardiac Troponin T (cTnT-wt)

Outcome

Isometric tension development and actomyosin Mg-ATPase activity as a function of Ca2+ concentrationsurrogate

Thr206 is identified as a functionally critical PKC phosphorylation residue on cardiac Troponin T that regulates myofilament tension and calcium sensitivity.

Cite This Study

Sumandea et al. (2003) studied this question.

synapsesocial.com/papers/6a70c8ad35aa2c282ce21b80 https://doi.org/10.1074/jbc.m306325200

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Identification of a Functionally Critical Protein Kinase C Phosphorylation Residue of Cardiac Troponin T

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Identification of a Functionally Critical Protein Kinase C Phosphorylation Residue of Cardiac Troponin T

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider