A simple colorimetric assay for measuring leucine-α-ketoglutarate transamination is described. It is reproducible, sensitive, and specific for α-ketoisocaproate. α-Ketoglutarate interferes only in that high concentrations decrease the color yield. This assay is suitable for detailed kinetic studies, even with crude tissue extracts, provided one corrects for this effect of α-ketoglutarate. A separate method was also described whereby transamination between isoleucine (or valine) and α-ketoglutarate can be measured, although this assay is not specific for the leucine transaminase in crude tissue extracts. The α-ketoisocaproate assay was used to examine subcellular fractions of pig heart muscle. Most of the activity was found in the soluble fraction.
No takes yet. Share an insight, caveat, or question.
Taylor et al. (1966) studied this question.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: