What question did this study set out to answer?

To characterize insulin-binding sites and determine the functional actions of insulin on primary neuronal cultures derived from rat brains.

December 1, 1985Journal of Biological ChemistryOpen Access

Insulin receptors and insulin modulation of norepinephrine uptake in neuronal cultures from rat brain.

View Full Paper

Ask AI

Bookmark

Authors

FBF T BoydUniversity of Minnesota DCDerrel W. ClarkeUniversity of Florida TMThomas F. MutherUniversity of Florida

Discussion

Member takes

Implication

In vitro laboratory study reveals insulin inhibits norepinephrine uptake in rat brain neuronal cultures, suggesting hormonal modulation of neural signaling.

Key Points

To characterize insulin-binding sites and determine the functional actions of insulin on primary neuronal cultures derived from rat brains.
Cultured primary neuronal cells from 1-day-old rat brains to assess specific 125I-insulin binding, kinetics, and competition with insulin analogues.
Performed Scatchard analysis to determine binding affinities and light microscopic autoradiography to localize receptor distribution.
Measured the functional effects of insulin incubation on 2-deoxyglucose and [3H]norepinephrine uptake in these cultures.
125I-insulin binding was 90% specific, reversible, and resolved into high-affinity (Kd = 11 nM) and low-affinity (Kd = 65 nM) binding sites located predominantly on neurites rather than the cell soma.
Insulin treatment had no effect on neuronal 2-deoxyglucose uptake, contrasting with previous findings in astrocyte glial cells.
Incubation with insulin resulted in a dose-dependent inhibition of [3H]norepinephrine uptake, achieving significant inhibition at concentrations as low as 1.67 x 10^-11 M.

Cite This Study

Boyd et al. (1985) studied this question.

synapsesocial.com/papers/6a70cf7987f921057127e7ac https://doi.org/10.1016/s0021-9258(17)36340-8

View Full Paper

Ask AI

Bookmark

Implication

In vitro laboratory study reveals insulin inhibits norepinephrine uptake in rat brain neuronal cultures, suggesting hormonal modulation of neural signaling.

Insulin receptors and insulin modulation of norepinephrine uptake in neuronal cultures from rat brain.

Discussion

Member takes

Implication

Key Points

Cite This Study

Insulin receptors and insulin modulation of norepinephrine uptake in neuronal cultures from rat brain.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider