What question did this study set out to answer?

To evaluate the capacity of differentiated bone marrow- and adipose-derived stem cells to express myelin components and model myelination when co-cultured with peripheral sensory neurons.

May 1, 2010Regenerative Medicine

Bone Marrow- and Adipose-Derived Stem Cells Show Expression of Myelin mRNAs and Proteins

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Authors

CMCristina MantovaniHumanitas University DMDaljeet MahayUniversity of Manchester PKPaul J. KinghamUmeå University

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Implication

In vitro study demonstrates myelin mRNA and protein expression in stem-cell-derived Schwann-like cells co-cultured with neurons, suggesting regenerative potential for peripheral nerve repair.

Key Points

To evaluate the capacity of differentiated bone marrow- and adipose-derived stem cells to express myelin components and model myelination when co-cultured with peripheral sensory neurons.
Differentiated adult bone marrow mesenchymal stem cells and adipose-derived stem cells into Schwann cell-like cells and co-cultured them with dissociated adult dorsal root ganglia neurons.
Assessed transcription and translation of myelin-specific markers using reverse transcription-PCR, western blotting, immunocytochemistry, and electron microscopy.
Transcripts for protein zero, peripheral myelin protein 22, and myelin basic protein were detected in differentiated stem cells following co-culture with neuronal cells.
Protein expression of protein zero, peripheral myelin protein 22, and myelin basic protein was verified in co-cultures through western blotting, immunostaining, and electron microscopy.

Cite This Study

Mantovani et al. (2010) studied this question.

synapsesocial.com/papers/6a70d72f439bab0cabc358cb https://doi.org/10.2217/rme.10.15

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Implication

In vitro study demonstrates myelin mRNA and protein expression in stem-cell-derived Schwann-like cells co-cultured with neurons, suggesting regenerative potential for peripheral nerve repair.

Bone Marrow- and Adipose-Derived Stem Cells Show Expression of Myelin mRNAs and Proteins

Discussion

Member takes

Implication

Key Points

Cite This Study

Bone Marrow- and Adipose-Derived Stem Cells Show Expression of Myelin mRNAs and Proteins

Discussion

Member takes

Implication

Key Points

Cite This Study

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