Why the study?
To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids in vitro.
A novel dual-modality imaging system combining OCT and fluorescence microscopy successfully monitors the 3D microstructure and calcium dynamics of human heart organoids in vitro.
Enables concurrent structural-electrophysiological imaging of heart organoids; extends preclinical tools while leaving clinical translation open.
To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids (hHOs), we developed a dual-modality imaging system combining the advantages of optical coherence tomography (OCT) and fluorescence microscopy. OCT provides high-resolution volumetric structural information, while fluorescence imaging indicates the electrophysiology of the hHOs' beating behavior. We verified that concurrent OCT motion mode (M-mode) and calcium imaging retrieved the same beating pattern from the heart organoids. We further applied dynamic contrast OCT (DyC-OCT) analysis to strengthen the verification and localize the beating clusters inside the hHOs. This imaging platform provides a powerful tool for studying and assessing hHOs in vitro, with potential applications in disease modeling and drug screening.
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Hao et al. (2023) studied this question.
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