What question did this study set out to answer?

To determine whether leptin stimulates endothelial nitric oxide release to modulate vascular tone and to identify the specific kinase phosphorylation pathways involved.

January 1, 2002DiabetesOpen Access

Leptin Effect on Endothelial Nitric Oxide Is Mediated Through Akt–Endothelial Nitric Oxide Synthase Phosphorylation Pathway

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Authors

CVCarmine VecchioneGeneral Cardiology AMAngelo MaffeiIstituto Neurologico Mediterraneo SCSalvatore ColellaIstituto Neurologico Mediterraneo

Discussion

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Implication

Experimental study demonstrates leptin triggers nitric oxide release and vasorelaxation in vascular tissues, indicating a PI3-kinase-independent Akt-eNOS signaling mechanism.

Key Points

To determine whether leptin stimulates endothelial nitric oxide release to modulate vascular tone and to identify the specific kinase phosphorylation pathways involved.
Measured direct nitric oxide release in endothelial cells and intact vessels using a fluorescent probe in the presence or absence of calcium and specific kinase inhibitors.
Evaluated vascular tone via aortic ring vasorelaxation and quantified Akt and endothelial nitric oxide synthase phosphorylation through immunoblotting.
Leptin stimulated nitric oxide release and induced aortic ring vasorelaxation, effects that were abolished by the tyrosine kinase inhibitor erbstatin A but unaffected by calcium removal or PI 3-kinase inhibitors.
Leptin promoted Akt phosphorylation and increased endothelial nitric oxide synthase phosphorylation at Ser1177 in both endothelial cells and vessels via an erbstatin A-sensitive pathway.

Cite This Study

Vecchione et al. (2002) studied this question.

synapsesocial.com/papers/6a70df09e5469ee92be18b78 https://doi.org/10.2337/diabetes.51.1.168

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Implication

Experimental study demonstrates leptin triggers nitric oxide release and vasorelaxation in vascular tissues, indicating a PI3-kinase-independent Akt-eNOS signaling mechanism.

Leptin Effect on Endothelial Nitric Oxide Is Mediated Through Akt–Endothelial Nitric Oxide Synthase Phosphorylation Pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

Leptin Effect on Endothelial Nitric Oxide Is Mediated Through Akt–Endothelial Nitric Oxide Synthase Phosphorylation Pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

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