Population
Insect cells (Sf9), HEK293 cells, COS cells, and murine skeletal muscles
Comparison
Recombinant adeno-associated virus type 5… vs rAAV5 produced in HEK293 cells
Design
Preclinical
Follow-up
1 week
Authors
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Sf9 rAAV5 production markedly boosts murine muscle transduction; extends scalable manufacturing options but leaves open human translation.
rAAV5 can be efficiently generated in insect cells, yielding vectors that may induce stronger transgene expression than those produced in mammalian cells due to differences in incorporated virion DNA.
Urabe et al. (2006) studied this question.
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