Triterpenoid saponins in roots of Achyranthese bidentata

Aim To study the constituents in the roots of Achyranthese bidentata Bl. Methods D-101 porous polymer resin column, normal-phase silica gel column chromatography, revised-phase medium pressure column chromatography, Sephadex LH-20 and prep-HPLC were used for the separation, and spectroscopic techniques were employed for the identification of the isolated compounds. Results Five saponins were isolated and elucidated as achyranthoside C ( 1 ), achyranthoside D ( 2 ), chikusetsusaponin IV ( 3 ), ginsenoside Ro ( 4 ) and zingibroside R 1 ( 5 ). Conclusion Compound 1 was obtained from natural source for the first time, and compound 3 was reported from the family of Amaranthaceae for the first time.