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The abbreviations used are: DNA polymerase, deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase EC 2.7.7.7; SDS, sodium dodecyl sulfate; KP,, potassium phosphate; PC buffer, 50 mM Tris-HCl (pH 7.6). 0.2 m dithiothreitol, 0.1 mM EDTA, 10% glycerol; DC buffer, 50 m~ Tris-HCI (pH 7.6), 0.1 mM EDTA, 1 mM dithiothreitol, 20% glycerol.
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Yamaguchi et al. (1980) studied this question.
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