The possibility of using liquid nitrogen storage conditions (-196°C) for the storage of grape (Vitis vinifera) pollen was demonstrated. Pollen from 21 cultivars was rapidly frozen and thawed. Germination tests were performed on 20% sucrose, 1% agar plates. No significant differences between unfrozen control samples and frozen pollen samples were found. Maximum differences in viability between control and frozen pollen were less than 5%.
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Parfitt et al. (1983) studied this question.