What question did this study set out to answer?

To classify normal and male-sterile maize cytoplasms by examining low molecular weight mitochondrial DNA banding profiles resolved by agarose gel electrophoresis.

June 1, 1980GeneticsOpen Access

Classification of Normal and Male-Sterile Cytoplasms in Maize. Ii. Electrophoretic Analysis of Dna Species in Mitochondria

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Authors

RKR. J. KembleKansas State University RGR. E. GunnKansas State University RFR. B. FlavellFamily Health International 360

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Implication

Comparative analysis reveals four distinct mitochondrial DNA profiles among male-sterile maize lines, indicating uniform cytoplasmic groups that align with nuclear fertility restoration.

Key Points

To classify normal and male-sterile maize cytoplasms by examining low molecular weight mitochondrial DNA banding profiles resolved by agarose gel electrophoresis.
Extracted mitochondrial DNA from N=31 maize lines possessing diverse cytoplasmic backgrounds within a uniform nuclear genome.
Analyzed low molecular weight mitochondrial DNA species using agarose gel electrophoresis to resolve banding variation.
Agarose gel electrophoresis resolved four distinct mitochondrial DNA banding patterns that correspond strictly to the N, T, S, and C cytoplasm groups designated by nuclear fertility restorer genes.
Classification of the 31 cytoplasmic sources assigned 6 lines to N, 4 to T, 19 to S, and 2 to C cytoplasm, showing complete concordance with mitochondrial translation product profiles.
Identified no within-group banding heterogeneity among lines belonging to the same cytoplasmic class, demonstrating uniform mitochondrial DNA species within each group.

Cite This Study

Kemble et al. (1980) studied this question.

synapsesocial.com/papers/6a70f7a7ac440176ef29a68d https://doi.org/10.1093/genetics/95.2.451

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Implication

Comparative analysis reveals four distinct mitochondrial DNA profiles among male-sterile maize lines, indicating uniform cytoplasmic groups that align with nuclear fertility restoration.

Classification of Normal and Male-Sterile Cytoplasms in Maize. Ii. Electrophoretic Analysis of Dna Species in Mitochondria

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Member takes

Implication

Key Points

Cite This Study

Classification of Normal and Male-Sterile Cytoplasms in Maize. Ii. Electrophoretic Analysis of Dna Species in Mitochondria

Discussion

Member takes

Implication

Key Points

Cite This Study

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