What question did this study set out to answer?

Determine whether the anti-inflammatory and protective effects of exogenous IL-10 and IL-13 in rat IgG immune complex-induced lung injury occur via inhibition of NF-kappa B activation.

November 15, 1997Journal of Clinical InvestigationOpen Access

In vivo suppression of NF-kappa B and preservation of I kappa B alpha by interleukin-10 and interleukin-13.

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Authors

ALAlex B. LentschApple (Israel)TSThomas P. ShanleyC. S. Mott Children's Hospital VSVidya SarmaUniversity of Michigan

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Implication

Animal study demonstrates suppression of NF-kappa B activation by IL-10 and IL-13 in lung injury models, indicating an anti-inflammatory mechanism via I kappa B alpha preservation.

Key Points

Determine whether the anti-inflammatory and protective effects of exogenous IL-10 and IL-13 in rat IgG immune complex-induced lung injury occur via inhibition of NF-kappa B activation.
Induced acute pulmonary injury in rats via the deposition of IgG immune complexes in the presence or absence of exogenous IL-10 or IL-13.
Assessed NF-kappa B nuclear translocation in alveolar macrophages and whole lung extracts using electrophoretic mobility shift assays.
Analyzed I kappa B alpha protein stability by Western blotting and mRNA expression levels using Northern blot analysis.
Both IL-10 and IL-13 suppressed the nuclear localization of NF-kappa B in alveolar macrophages and whole lung tissues following IgG immune complex challenge.
Treatment with IL-10 or IL-13 preserved I kappa B alpha protein levels and augmented I kappa B alpha mRNA expression in injured lungs.

Cite This Study

Lentsch et al. (1997) studied this question.

synapsesocial.com/papers/6a70f96735aa2c282ce24d8a https://doi.org/10.1172/jci119786

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Implication

Animal study demonstrates suppression of NF-kappa B activation by IL-10 and IL-13 in lung injury models, indicating an anti-inflammatory mechanism via I kappa B alpha preservation.

In vivo suppression of NF-kappa B and preservation of I kappa B alpha by interleukin-10 and interleukin-13.

Discussion

Member takes

Implication

Key Points

Cite This Study

In vivo suppression of NF-kappa B and preservation of I kappa B alpha by interleukin-10 and interleukin-13.

Discussion

Member takes

Implication

Key Points

Cite This Study

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