Hemoglobin Synthesis in Rabbit Reticulocytes in Vitro

We have reported the rapid incorporation of labeled amino acids into proteins of rabbit reticulocytes in vitro and the stimulation of this process certain amino acids, iron, fructose-amino acids, glucose, and some, yet, unidentified material in the filtrate of boiled plasma. The incorporation measured in the mixture of the total proteins of the reticulocytes (1, 2). the study reported here two protein fractions were isolated, the hemoglobin, comprises more than 75 per cent of the total, and the water-insoluble . The hemoglobin was fractionated into heme and globin. were carried out with four C^(14)-labeled amino acids and with stimulators to ascertain whether heme synthesis and amino acid went parallel or not.