October 13, 2010Journal of General VirologyOpen Access

Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1

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Population

Mouse L929 cells stably expressing human P-selectin glycoprotein ligand-1 infected with five original…

Comparison

Propagation of EV71 strains in L-PSGL-1 cells to… vs Original EV71 strains

Design

Preclinical

Authors

KMKikuko MiyamuraJikei University School of Medicine YNYorihiro NishimuraNational Institute of Infectious Diseases MAMasahiro AboJikei University School of Medicine

Discussion

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Implication

No immediate clinical implications from L-PSGL-1 cell adaptation; leaves open VP2-149 relevance in animal or human EV71 models.

Structured PICO

Population

Mouse L929 cells stably expressing human P-selectin glycoprotein ligand-1 (L-PSGL-1 cells) infected with five original enterovirus 71 (EV71) strains

Intervention

Propagation of EV71 strains in L-PSGL-1 cells to generate EV71 variants (EV71-LPS)

Comparator

Original EV71 strains

Outcome

Replication kinetics and entire genomic sequence comparison to identify adaptive mutationssurrogate

An adaptive mutation at VP2-149, along with a PSGL-1-binding phenotype, may facilitate efficient PSGL-1-dependent replication of EV71 strains in L-PSGL-1 cells.

Cite This Study

Miyamura et al. (2010) studied this question.

synapsesocial.com/papers/6a70feebe5469ee92be1a479 https://doi.org/10.1099/vir.0.022418-0

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Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider