Why the study?
The mechanisms underlying the strong antitumor effect of carbon-ion radiotherapy, especially regarding metabolic alterations in cancer cells, were not fully elucidated.
Does carbon-ion irradiation alter metabolite levels in human cancer cell lines?
Does carbon-ion irradiation alter metabolite levels in human cancer cell lines?
Carbon-ion irradiation up-regulates TCA cycle intermediates and 2-hydroxyglutaric acid in cancer cells, providing insight into its metabolic effects.
Answers are grounded in this paper and cite their sources.
Metabolic shifts in carbon-ion-treated cell lines are hypothesis-generating; in vivo and clinical studies needed before any translation.
BACKGROUND/AIM: Carbon-ion radiotherapy has strong antitumor effects in X-ray-resistant tumors. However, the mechanisms underlying the strong antitumor effect, especially on metabolic alterations, are not fully elucidated. This study aimed to determine the effect of therapeutic carbon ions on metabolic alterations in cancer cells. MATERIALS AND METHODS: Five human cancer cell lines were used in this study. The change in 333 metabolite levels in response to carbon ions was analyzed using gas chromatography-mass spectrometry. RESULTS: Fifty-two metabolites were commonly detected in all cell lines. The levels of five metabolites significantly changed in four or more cell lines. Three of the five metabolites (i.e., 2-ketoglutaric acid, fumaric acid and 2-hydroxyglutaric acid) were associated with the TCA cycle. TCA cycle intermediates and the downstream oncometabolite 2-hydroxyglutaric acid were up-regulated by carbon ions. CONCLUSION: We demonstrated for the first time that TCA cycle intermediates and 2-hydroxyglutaric acid are up-regulated after carbon-ion irradiation.
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Osu et al. (2021) studied this question.
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