In vitro Propagation and Determination of Total Phenolic Compounds, Flavonoid Contents and Antioxidative Activity of Globba globulifera Gagnep

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In vitro shoot regeneration Surface-sterilization procedures and initial culture establishmentThe bulbils of G. globulifera from plants growing under a natural environment were collected from Udon Thani Province, Thailand.The bulbils were washed for 30 min under running tap water, soaked for 1 min in 70% (v/v) ethanol, followed by ABSTRACT Introduction: Currently, there is a reduction in the number of Globba globulifera, which is due to its slow multiplication rate, high susceptibility to pathogenic diseases and overexploitation of the plant from natural sources.In vitro culture to study suitable concentrations of plant growth regulators for shoot and root induction of G. globulifera.Bioactive compounds were measured by TPC, TFC and FRSA methods for comparison of those from in vitro and natural conditions.Methods: Microshoots were cultured on solid and liquid MS medium supplemented with various concentrations of cytokinins (BA, Kinetin and TDZ) and auxins (NAA and IBA) for eight weeks.Methanol was used as the extraction solvent via the ultrasonic method, TPC and TFC were both measured.DPPH for free radical scavenging activity was investigated.Results: The best result for shoot formation was achieved when culturing on MS medium with 3 mg/l and 5 mg/l of BAP or 5 mg/l of BAP plus 1 mg/l of IBA.The plantlets were transplanted to pots in a greenhouse.All the planting material showed a 100% survival rate.The rhizomes of in vitroderived plantlets showed the highest value of TPC (52.28 mg GAE/g crude extract) and FRSA (93.55%) and lowest of IC 50 (0.46 mg/ml).Conclusion: The in vitro culture and antioxidant activity analysis could be the foundation for plant propagation in large quantities and the use of medicine.