What question did this study set out to answer?

To describe standardized propagation protocols for staphylococcal typing bacteriophages and evaluate their capacity to differentiate diverse strains of Staphylococcus aureus.

September 1, 1952Journal of HygieneOpen Access

Bacteriophage typing ofstaphylococcus aureus

View Full Paper

Ask AI

Bookmark

Authors

RWR. E. O. WilliamsGenomics England JRJoan E. RipponNational Public Health Laboratory

Discussion

Member takes

Implication

Laboratory study reveals distinct bacteriophage lysis patterns across 567 Staphylococcus aureus strains, indicating reproducible frameworks for epidemiological classification.

Key Points

To describe standardized propagation protocols for staphylococcal typing bacteriophages and evaluate their capacity to differentiate diverse strains of Staphylococcus aureus.
Propagated typing phages across fluid media (nutrient broth, Todd-Hewitt broth, glucose-peptone-water) and agar, while evaluating the effects of culture age and inoculation procedures.
Tested propagated phage filtrates for purity, titre stability, and non-specific bacterial growth-inhibiting agents.
Conducted bacteriophage typing on N=567 independent strains of staphylococci using routine test dilutions and undiluted filtrates.
Approximately 40% of staphylococci were not lysed at routine test dilutions, though approximately half of these untypable strains were successfully lysed by undiluted phage filtrates.
Of 567 tested strains, 229 exhibited strong lysis across 82 distinct phage patterns, whereas only 118 strains were lysed strongly by a single phage.
Associated lysis reactions segregated the phages into three primary groups designated as '3A', '6/47', and '52'.

Cite This Study

Williams et al. (1952) studied this question.

synapsesocial.com/papers/6a710feee5469ee92be1b179 https://doi.org/10.1017/s002217240001963x

View Full Paper

Ask AI

Bookmark

September 1, 1952Journal of HygieneOpen Access

Bacteriophage typing ofstaphylococcus aureus

View Full Paper

Ask AI

Bookmark

Authors

RWR. E. O. WilliamsGenomics England JRJoan E. RipponNational Public Health Laboratory

Discussion

Member takes

Implication

Laboratory study reveals distinct bacteriophage lysis patterns across 567 Staphylococcus aureus strains, indicating reproducible frameworks for epidemiological classification.

Key Points

To describe standardized propagation protocols for staphylococcal typing bacteriophages and evaluate their capacity to differentiate diverse strains of Staphylococcus aureus.
Propagated typing phages across fluid media (nutrient broth, Todd-Hewitt broth, glucose-peptone-water) and agar, while evaluating the effects of culture age and inoculation procedures.
Tested propagated phage filtrates for purity, titre stability, and non-specific bacterial growth-inhibiting agents.
Conducted bacteriophage typing on N=567 independent strains of staphylococci using routine test dilutions and undiluted filtrates.
Approximately 40% of staphylococci were not lysed at routine test dilutions, though approximately half of these untypable strains were successfully lysed by undiluted phage filtrates.
Of 567 tested strains, 229 exhibited strong lysis across 82 distinct phage patterns, whereas only 118 strains were lysed strongly by a single phage.
Associated lysis reactions segregated the phages into three primary groups designated as '3A', '6/47', and '52'.

Cite This Study

Williams et al. (1952) studied this question.

synapsesocial.com/papers/6a710feee5469ee92be1b179 https://doi.org/10.1017/s002217240001963x

Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark