Mung Bean Nuclease I

Partial heat denaturation of preparations of DNA of different origin produced regions that became susceptible to mung bean nuclease I.These regions were distributed throughout the whole molecule of DNA.The susceptible regions were richer in A and T than the over-all composition of the parent DNA, regardless of whether the parent was A + T-rich or C + G-rich DNA.In the digested material C = G, A = T, therefore both strands were attacked simultaneously.