The role of LPD-nanoparticles containing recombinant major surface glycoprotein ofLeishmania(rgp63) in protection against leishmaniasis in murine model

Hengameh Firouzmand; Mehrnosh Sahranavard; Ali Badiee; Ali Khamesipour; Seyedeh Hoda Alavizadeh; Afshin Samiei; Dina Soroush; Masoumeh Tavassoti Kheiri; Fereidoun Mahboudi; Mahmoud Reza Jaafari

doi:10.1080/08923973.2017.1407941

December 6, 2017Immunopharmacology and Immunotoxicology

The role of LPD-nanoparticles containing recombinant major surface glycoprotein ofLeishmania(rgp63) in protection against leishmaniasis in murine model

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Key Points

To assess whether recombinant glycoprotein 63 (rgp63) delivered via liposome-polycation-DNA (LPD) nanoparticles stimulates protective cell-mediated immunity against Leishmania major in BALB/c mice.
Encapsulated leishmania promastigote surface antigen rgp63 into nano-sized LPD complexes adjuvanted with CpG DNA.
Immunized BALB/c mice three times with LPD (CpG)-rgp63, rgp63-CpG DNA, empty LPD (CpG), unformulated rgp63, or 5% dextrose vehicle controls prior to L. major parasite challenge.
Assessed vaccine efficacy by measuring footpad swelling, splenic parasite burdens, antigen-specific IgG subclasses, and IFN-γ production by reactive T lymphocytes.
Mice immunized with LPD (CpG)-rgp63 exhibited minimal footpad swelling and the lowest splenic parasite burdens after infection compared with all control groups.
The LPD (CpG)-rgp63 formulation elicited superior cell-mediated and humoral immune responses, characterized by elevated post-challenge IgG2a antibody titers and heightened IFN-γ secretion.

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Implication

Preclinical study demonstrates enhanced immune protection against Leishmania major in vaccinated mice, highlighting LPD nanoparticles as a promising vaccine delivery platform.

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Firouzmand et al. (2017) studied this question.

synapsesocial.com/papers/6a711370f44fa9f079ded886 https://doi.org/10.1080/08923973.2017.1407941

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The role of LPD-nanoparticles containing recombinant major surface glycoprotein ofLeishmania(rgp63) in protection against leishmaniasis in murine model

Key Points

No abstract yet

Implication

Discussion

Cite This Study

The role of LPD-nanoparticles containing recombinant major surface glycoprotein ofLeishmania(rgp63) in protection against leishmaniasis in murine model

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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