What question did this study set out to answer?

To synthesize and biophysically characterize the defined 30-base-pair duplex block polymer d(C15A15)·d(T15G15) using sequential enzymatic reactions.

November 1, 1974Journal of Biological ChemistryOpen Access

Synthesis and Characterization of the Duplex Block Polymer d(C15A15)·d(T15G15)

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Authors

JBJohn F. BurdCité des Sciences et de l'Industrie RWRobert D. WellsUniversity of Bern

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Implication

Biochemical analysis demonstrates multi-step enzymatic synthesis of defined duplex block polymers, indicating reliable methods for assembling custom oligonucleotides.

Key Points

To synthesize and biophysically characterize the defined 30-base-pair duplex block polymer d(C15A15)·d(T15G15) using sequential enzymatic reactions.
Synthesized strands through sequential enzymatic steps using pancreatic DNase, calf thymus terminal transferase, and T4 DNA polymerase.
Incorporated [14C]dAMP into the d(C15A15) strand and [3H]dGMP into the d(T15G15) strand to assess chain length and purity using analytical and preparative gel electrophoresis.
Characterized polymer properties using ultraviolet absorbance spectroscopy, circular dichroism, and analytical and preparative sedimentation centrifugation.
Gel electrophoresis successfully separated and resolved individual synthesized oligonucleotides up to 50-mer chain lengths.
Ultraviolet absorbance and circular dichroism profiles matched the additive and averaged spectra of d(A)n·d(T)n and d(G)n·d(C)n homopolymers.
Sedimentation centrifugation confirmed the expected molecular size, hydrodynamic properties, and duplex block structure.

Cite This Study

Burd et al. (1974) studied this question.

synapsesocial.com/papers/6a711a58fe4101aa97e06f20 https://doi.org/10.1016/s0021-9258(19)42079-6

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Implication

Biochemical analysis demonstrates multi-step enzymatic synthesis of defined duplex block polymers, indicating reliable methods for assembling custom oligonucleotides.

Synthesis and Characterization of the Duplex Block Polymer d(C15A15)·d(T15G15)

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Implication

Key Points

Cite This Study

Synthesis and Characterization of the Duplex Block Polymer d(C15A15)·d(T15G15)

Discussion

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Implication

Key Points

Cite This Study

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