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November 1, 1985Journal of Biological ChemistryOpen Access

Hepatic retinol metabolism. Distribution of retinoids, enzymes, and binding proteins in isolated rat liver cells.

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Authors

RBRune BlomhoffOslo University HospitalMRMagnhild RasmussenOslo University HospitalÅNÅ. NilssonLTT Bio-Pharma (Japan)

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Implication

Experimental study reveals differential retinol and enzyme distribution across rat liver cell types, indicating a central role for stellate cells in vitamin A storage.

Key Points

  • To quantify the distribution and cellular concentration of retinoids, metabolizing enzymes, and intracellular binding proteins across distinct isolated rat liver cell populations.
  • Isolated specific rat liver cell fractions, including perisinusoidal stellate cells, parenchymal cells, endothelial cells, and Kupffer cells.
  • Quantified retinoid concentrations, retinyl palmitate hydrolase and acyl-CoA:retinol acyltransferase activities, and cellular retinoid-binding proteins across isolated cell preparations.
  • Perisinusoidal stellate cells contained 28 to 34 nmol of retinoids per 10^6 cells (over 98% retinyl esters), accounting for 80% or more of total hepatic retinoids, compared with 0.5 to 0.8 nmol per 10^6 cells in parenchymal cells.
  • Parenchymal cells contained 75% to 80% of total hepatic retinyl palmitate hydrolase and acyl-CoA:retinol acyltransferase activities and over 90% of total cellular retinol binding protein, but stellate cells displayed markedly higher specific activity and a 22-fold higher concentration of cellular retinol binding protein per unit protein.
  • Isolated endothelial and Kupffer cells contained very low levels of retinoids, while stellate cells exhibited distinct enrichment of cellular retinoic acid binding protein.

Cite This Study

Blomhoff et al. (1985) studied this question.

synapsesocial.com/papers/6a711d04fe4101aa97e0706dhttps://doi.org/10.1016/s0021-9258(17)38759-8
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Also Consider

Synapse has enriched one closely related paper. Consider it for comparative context:

  1. 1Cellular retinol-binding protein from rat liver. Purification and characterization.1978 · 209 citations