The metabolism of testosterone-4- 14 C by rat liver slices has been evaluated with and without the presence of diphenylhydantoin (DPH). Radioactive metabolites were identified by paper chromatography and recrystallization to constant specific activities with appropriate standards. With female rat liver slice incubation, under the conditions utilized, androsterone (A) and allo-tetrahydrotestosterone (allo-THT) is increased 4–5 fold by the presence of DPH. In the male, aetiocholanolone (E) and tetrahydrotestosterone (THT) formation is noted with increased conversion to these in the presence of DPH. It is postulated that the DPH effect may be due to competitive inhibition of hepatic microsomal hydroxylase activity while allowing for unabated Δ 4 -reductase action.
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Sholiton et al. (1967) studied this question.