An Autoimmolative Spacer Allows First‐Time Incorporation of a Unique Solid‐State Fluorophore into a Detection Probe for Acyl Hydrolases

Mind your HPQs: A probe to detect specific peptidases based on the unique solid-state fluorophore HPQ has been developed by the use of an autoimmolative cyclization spacer in conjunction with an acylated O,O-acetal linked to HPQ (see scheme). Under our assay conditions this probe detected the enzyme with a minimum half-time of only 560 s.