Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.

Patrick K. Umeda; A M Sinha; Smilja Jakovcic; S. Merten; H J Hsu; K. N. Subramanian; R Zak; Murray Rabinowitz

doi:10.1073/pnas.78.5.2843

May 1, 1981Proceedings of the National Academy of SciencesOpen Access

Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.

View Full Paper

Ask AI

Bookmark

Share

Ask about this paper

Answers are grounded in this paper and cite their sources.

Abstract

Recombinant DNA clones containing sequences for two different types of myosin heavy chain (HC) genes from chicken embryonic skeletal muscle were constructed and analyzed. Specificity of the clones for myosin HC was demonstrated by hybrid-arrested translation, by hybridization to a 7.0-kb mRNA, and by comparison of DNA sequences with known amino acid sequences of rabbit skeletal muscle myosin HC. Restriction enzyme and electron-microscopic heteroduplex analysis showed the presence of two distinct but homologous cDNA sequences. Hybrid melting curves indicated that both types of sequences represent fast myosin HC sequences.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Umeda et al. (1981) studied this question.

synapsesocial.com/papers/6a712c27e36a167817e30123 https://doi.org/10.1073/pnas.78.5.2843

View Full Paper

Ask AI

Bookmark

Share

Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.

Ask about this paper

Abstract

Discussion

Cite This Study

Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.

Ask about this paper

Abstract

Discussion

Cite This Study

Keep reading

Keep reading