What question did this study set out to answer?

To evaluate the validity of substrate uptake kinetics for measuring microbial heterotrophic activity in marine environments and estimate organic carbon assimilation using labeled glucose.

April 1, 1966Limnology and OceanographyOpen Access

Studies on Heterotrophic Activity in Seawater Based on Glucose Assimilation1

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Authors

RVRalph F. VaccaroBermuda Institute of Ocean Sciences HJHolger W. JannaschNew England Biolabs (United States)

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Implication

Biogeochemical analysis demonstrates microbial glucose uptake kinetics in seawater, indicating that bioassays can quantify natural substrate assimilation.

Key Points

To evaluate the validity of substrate uptake kinetics for measuring microbial heterotrophic activity in marine environments and estimate organic carbon assimilation using labeled glucose.
Calculated minimum in situ organic carbon uptake rates across coastal and offshore seawater samples using carbon-14-labeled glucose.
Developed a complementary bioassay using a marine bacterium resembling Achromobacter aquamarinus capable of detecting glucose concentrations down to 4 × 10⁻⁸ M.
Demonstrated measurable shifts in heterotrophic uptake kinetics, distinctly reflecting the influence of nutrient-poor Amazon River water in offshore South Atlantic regions.
Confirmed the feasibility of pairing natural community uptake measurements with pure-culture bioassays, while establishing the ongoing need for improved direct substrate quantification.

Cite This Study

Vaccaro et al. (1966) studied this question.

synapsesocial.com/papers/6a712c6726770c2b8de0f765 https://doi.org/10.4319/lo.1966.11.4.0596

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Implication

Biogeochemical analysis demonstrates microbial glucose uptake kinetics in seawater, indicating that bioassays can quantify natural substrate assimilation.

Studies on Heterotrophic Activity in Seawater Based on Glucose Assimilation1

Discussion

Member takes

Implication

Key Points

Cite This Study

Studies on Heterotrophic Activity in Seawater Based on Glucose Assimilation1

Discussion

Member takes

Implication

Key Points

Cite This Study

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