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To determine the role of the phyllosphere microbiome in conferring resistance against the fungal pathogen Botrytis cinerea in Arabidopsis cuticle mutants.

January 4, 2016New PhytologistOpen Access

The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen

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To determine the role of the phyllosphere microbiome in conferring resistance against the fungal pathogen Botrytis cinerea in Arabidopsis cuticle mutants.
Inoculated wild-type Arabidopsis, bdg mutants, and lacs2.3 mutants with Botrytis cinerea under sterile versus non-sterile growth conditions.
Profiled phyllosphere bacterial communities across genotypes using 16S ribosomal DNA sequencing and isolated individual bacterial strains using high-coverage sequencing.
Tested pathogen protection following re-inoculation of leaf microbiome washes and isolated Pseudomonas sp. onto Arabidopsis leaves and apple fruits.
Cuticle mutant bdg lost fungal resistance under sterile conditions but regained full resistance upon reintroduction of phyllosphere microbiome washes, whereas lacs2.3 maintained resistance independently.
16S rDNA profiling revealed distinct phyllosphere bacterial community compositions on cuticle mutants compared with wild-type plants.
An isolated Pseudomonas sp. from bdg leaves conferred resistance against B. cinerea on both Arabidopsis and apple fruits through plant-dependent mechanisms without exhibiting direct antifungal activity.

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Implication

Experimental study reveals leaf surface microbiome-mediated resistance against Botrytis cinerea in Arabidopsis cuticle mutants, highlighting plant-dependent biocontrol by commensal bacteria.

Cite This Study

Ritpitakphong et al. (2016) studied this question.

synapsesocial.com/papers/6a7131e935aa2c282ce28154 https://doi.org/10.1111/nph.13808

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Implication

Experimental study reveals leaf surface microbiome-mediated resistance against Botrytis cinerea in Arabidopsis cuticle mutants, highlighting plant-dependent biocontrol by commensal bacteria.

The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen

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Discussion

Implication

Cite This Study

The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen

Key Points

Discussion

Implication

Cite This Study

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