What question did this study set out to answer?

To establish and validate a competitive repopulation assay for measuring the long-term functional capacity of hemopoietic stem cells.

January 1, 1980BloodOpen Access

Competitive repopulation: a new assay for long-term stem cell functional capacity

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Authors

DHDE HarrisonJackson Laboratory

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Implication

Experimental study demonstrates a linear relationship between donor marrow ratios and hemoglobin production in irradiated mice, indicating a reliable assay for stem cell functional capacity.

Key Points

To establish and validate a competitive repopulation assay for measuring the long-term functional capacity of hemopoietic stem cells.
Mixed test donor bone marrow cells with a constant number of competitor marrow cells carrying a distinguishable hemoglobin marker across varying cell ratios from 0:1 to 3:1.
Injected marrow mixtures into irradiated, stem-cell-depleted recipient mice across multiple strain combinations (B6 with WBB6F1, WB with WBB6F1, and CBA with B6CBAF1).
Donor-derived hemoglobin percentages increased in a direct, linear relationship as the ratio of test donor to competitor marrow cells increased from 0:1 to 3:1.
Correlation coefficients between donor cell input ratio and donor hemoglobin output ranged from 0.78 to 0.98 in single experiments, and 0.68 to 0.75 across pooled experimental data.

Cite This Study

DE Harrison (1980) studied this question.

synapsesocial.com/papers/6a713bd884cc45bb7bdfe7dc https://doi.org/10.1182/blood.v55.1.77.77

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Implication

Experimental study demonstrates a linear relationship between donor marrow ratios and hemoglobin production in irradiated mice, indicating a reliable assay for stem cell functional capacity.

Competitive repopulation: a new assay for long-term stem cell functional capacity

Discussion

Member takes

Implication

Key Points

Cite This Study

Competitive repopulation: a new assay for long-term stem cell functional capacity

Discussion

Member takes

Implication

Key Points

Cite This Study

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