bla VIM-2 and bla VIM-7 Carbapenemase-Producing Pseudomonas aeruginosa Isolates Detected in a Tertiary Care Medical Center in the United States: Report from the MYSTIC Program

Halim Aboufaycal; Hélio S. Sader; K. V. I. Rolston; Lalitagauri M. Deshpande; Mark A. Toleman; Gerald P. Bodey; Issam Raad; Ronald N. Jones

doi:10.1128/jcm.01351-06

December 7, 2006Journal of Clinical MicrobiologyOpen Access

bla VIM-2 and bla VIM-7 Carbapenemase-Producing Pseudomonas aeruginosa Isolates Detected in a Tertiary Care Medical Center in the United States: Report from the MYSTIC Program

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Key Points

To identify and characterize the genetic mechanisms underlying extreme multidrug resistance in clinical Pseudomonas aeruginosa isolates collected through a national surveillance program.
Assayed two multidrug-resistant Pseudomonas aeruginosa isolates (N=2) from a tertiary cancer center within the Meropenem Yearly Susceptibility Test Information Collection program for antimicrobial susceptibility.
Conducted metallo-beta-lactamase phenotypic screening, followed by polymerase chain reaction and DNA sequence analysis targeting blaVIM genes.
Evaluated isolate relatedness and epidemiological clonality through automated ribotyping and pulsed-field gel electrophoresis.
Both isolates showed broad-spectrum resistance across beta-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and carbapenems, retaining susceptibility solely to polymyxin B (MIC ≤ 2 μg/ml).
Sequencing identified the metallo-beta-lactamase genes blaVIM-7 and blaVIM-2 in the respective isolates.
Ribotyping and pulsed-field gel electrophoresis revealed distinct molecular profiles, establishing that the two strains emerged independently across different times and clinical locations.

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Implication

Surveillance report reveals multidrug-resistant Pseudomonas aeruginosa carrying VIM carbapenemase genes in a hospital setting, highlighting the independent emergence of diverse resistant strains.

Abstract

Two Pseudomonas aeruginosa strains resistant to beta-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and carbapenems and susceptible only to polymyxin B (MIC <or= 2 microg/ml) were identified as part of the Meropenem Yearly Susceptibility Test Information Collection program. Metallo-beta-lactamase screening tests were positive, PCR yielded products with blaVIM primers, and sequence analysis revealed blaVIM-7 and blaVIM-2. The isolates had distinct ribotype and pulsed-field gel electorphoresis patterns and appeared independently, remote in time and location, at the same cancer center.

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Aboufaycal et al. (2006) studied this question.

synapsesocial.com/papers/6a713c3aac440176ef29e952 https://doi.org/10.1128/jcm.01351-06

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bla VIM-2 and bla VIM-7 Carbapenemase-Producing Pseudomonas aeruginosa Isolates Detected in a Tertiary Care Medical Center in the United States: Report from the MYSTIC Program

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bla VIM-2 and bla VIM-7 Carbapenemase-Producing Pseudomonas aeruginosa Isolates Detected in a Tertiary Care Medical Center in the United States: Report from the MYSTIC Program

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