November 1, 1999Biochemistry

The Ends of Tropomyosin Are Major Determinants of Actin Affinity and Myosin Subfragment 1-Induced Binding to F-Actin in the Open State

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Population

Rat alpha-tropomyosin variants expressed in Escherichia coli

Comparison

Different combinations of N- and C-terminal… vs Comparison among different tropomyosin variants

Design

Preclinical

Authors

JMJoanna MoraczewskaKazimierz Wielki University in Bydgoszcz KNKelley Nicholson-FlynnJohnson University SHSarah E. Hitchcock‐DeGregoriRutgers, The State University of New Jersey

Discussion

Member takes

Implication

Tropomyosin terminal sequences govern actin affinity and myosin cooperativity; hypothesis-generating for thin-filament regulation, leaves clinical translation open.

Structured PICO

Population

Rat alpha-tropomyosin variants expressed in Escherichia coli

Intervention

Different combinations of N- and C-terminal sequences encoded by exons 1a or 1b and exons 9a, 9c or 9d

Comparator

Comparison among different tropomyosin variants

Outcome

Actin affinity and the cooperativity with which myosin S1 promotes binding of tropomyosin to actin in the open statesurrogate

The specific N- and C-terminal sequences of tropomyosin determine its actin affinity, which in turn dictates the cooperativity of myosin S1-induced tropomyosin binding to actin.

Cite This Study

Moraczewska et al. (1999) studied this question.

synapsesocial.com/papers/6a7142de5d37378ac1de6a79 https://doi.org/10.1021/bi991816j

View Full Paper

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Implication

Tropomyosin terminal sequences govern actin affinity and myosin cooperativity; hypothesis-generating for thin-filament regulation, leaves clinical translation open.

The Ends of Tropomyosin Are Major Determinants of Actin Affinity and Myosin Subfragment 1-Induced Binding to F-Actin in the Open State

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The Ends of Tropomyosin Are Major Determinants of Actin Affinity and Myosin Subfragment 1-Induced Binding to F-Actin in the Open State

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider