December 29, 2009BiochemistryOpen Access

Modification of Loop 1 Affects the Nucleotide Binding Properties of Myo1c, the Adaptation Motor in the Inner Ear

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Structured PICO

Population

Insect cells expressing mutants of a truncated form of Myo1c, Myo1c(1IQ), and chimeras

Intervention

Modification of loop 1 (replacement with alanines, substitution with loop 1 from tonic smooth muscle myosin II, phasic muscle myosin II, or a single glycine)

Comparator

Wild-type Myo1c(1IQ) or Myo1c(SAH)-WT

Outcome

ATPase activity, transient kinetics properties, Ca(2+) sensitivity, ADP release rate, and actin translocation speedsurrogate

Modification of loop 1 in Myo1c alters its nucleotide binding properties and motility, but the changes do not resemble the behavior of loop donor myosins.

Discussion

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Implication

Loop 1 swaps in Myo1c alter kinetics without donor-like behavior; leaves open the structural basis of myosin isoform specificity.

Cite This Study

Adamek et al. (2009) studied this question.

synapsesocial.com/papers/6a71437efe4101aa97e07fc1 https://doi.org/10.1021/bi901803j

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Modification of Loop 1 Affects the Nucleotide Binding Properties of Myo1c, the Adaptation Motor in the Inner Ear

Structured PICO

Discussion

Implication

Cite This Study

Modification of Loop 1 Affects the Nucleotide Binding Properties of Myo1c, the Adaptation Motor in the Inner Ear

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider