A transferase from Clostridium sticklandii catalyzes the formation of a new 5'-adenylic acid by reaction between adenine and β-nicotinate mononucleotide. A method for purification of the nucleotide on Dowex 1 is described. Although the composition and the ionophoretic behavior of the compound correspond to those of AMP, the chromatographic, optical, and stability properties do not. Evidence is presented for assigning to this nucleotide the structure 7-α-d-ribofuranosyladenine 5'-phosphate. The substance is a likely precursor of pseudovitamin B12.
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Friedmann et al. (1969) studied this question.
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