Alteration of Collagen Content and Macrophage Distribution in White Adipose Tissue under the Influence of Maternal and Postnatal Diet in Male Rat Offspring

Figures

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  Figure 1. Schematic flowchart of the experiment and duration of control and high-fat diet in mothers and offspring of Sprague Dawley rats.

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  Figure 2. Collagen content (%) in: (a) subcutaneous, (b) epididymal and (c) perirenal adipose tissue; with statistical significance between groups. Data are presented using Whiskers bar graphs (mean, 5–95 percentile). Post hoc LSD test was used to test two independent groups, * p < 0.05 was considered significant. CD—standard laboratory chow, HFD—high-fat diet.

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  Figure 3. Representative images of collagen staining with Mallory’s trichrome staining in subcutaneous adipose tissue (collagen is visible in green color) of the groups of male rat offspring: (a) CD-CD group, (b) CD-HFD group, (c) HFD-CD group, and (d) HFD-HFD group. Magnification: 200×. Scale bar: 200 µm. CD—standard laboratory chow, HFD—high-fat diet.

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  Figure 4. Representative images of CD163+ cells (arrow) in perirenal adipose tissue: (a) CD-CD group, (b) CD-HFD group, (c) HFD-CD group, and (d) HFD-HFD group of male rat offspring. Magnification: 200×. Scale bar: 200 µm.

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  Figure 5. Polarization of macrophage markers among groups of offspring where the number of CD163+ cells is presented relative to the number of CD68+ cells in: (a) subcutaneous, (b) epididymal and (c) perirenal adipose tissue, with statistical significance comparing groups. Data are presented using Whiskers bar graphs (mean, 5–95 percentile). Post hoc LSD test was used for testing two independent groups, * p < 0.05 was considered significant. CD—standard laboratory chow, HFD—high-fat diet.