Fate of a toxic Microcystis aeruginosa bloom introduced into a subtropical estuary from a flow-managed canal and management implications

The Caloosahatchee Estuary in southwest Florida, USA , is regularly subject to the introduction of toxic Microcystis aeruginosa blooms, often originating from the eutrophic Lake Okeechobee via the C-43 Canal. The focus of this study was to determine the responses of one of these introduced blooms to progressively elevated salinity levels as the bloom water mass moved through the estuary. In the upper estuary, salinities were freshwater, and surface blooms of large colonies of M. aeruginosa were observed, along with peak microcystin toxin concentrations up to 107 μg L −1 , all in the particulate fraction. In the mid-estuary, salinity levels increased to 2–6, and surface blooms were again observed, with peak microcystin concentrations up to 259 μg L −1 , however, significant levels of extracellular toxin were also observed (i.e., 17.8 μg L −1 ), suggesting a level of osmotic stress on M. aeruginosa . In the lower estuary, salinities ranged from 6 to 25 and very few viable M. aeruginosa colonies were observed, but significant levels of extracellular microcystin (i.e., 0.5 μg L −1 ) were present throughout the water column. It is noteworthy that average total microcystin concentrations in the water column (i.e., particulate + extracellular) remained constant throughout the movement of the bloom water mass during its transit through the estuary, revealing the negligible rate of microcystin degradation during the ten-day transit. The results also provide insights into the changes in the distribution of particulate and extracellular microcystin along the salinity gradient, which has implications for management of risks for ecosystem and human health, and how these risks may be affected by management of releases from three water control structures in the C-43 Canal. Discharge rates from the water control structures play major roles in the rate of movement of blooms through the C-43 Canal-Caloosahatchee Estuary ecosystem. The potential implications of discharge regulation for the management of M. aeruginosa in the ecosystem are discussed from the perspectives of blooms of allochthonous and autochthonous origin.