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The principal lipid of A. agilis, A. tumefaciens, and E. coli extractable with ethanol and methanol-chloroform was a phosphatidyl ethanolamine. A phosphatidyl choline was detectable only in A. tumefaciens. The fatty acids of phospholipid from A. agilis were found to be myristic (7%), palmitic (35%), palmitoleic (41%), and octadecenoic (17%) acids. The fatty acids from A. tumefaciens were myristic (l%), palmitic (15%), hexadecenoic (l%), methylene hexadecanoic (6%), octadecenoic (30%), and lactobacillic (47%) acids. The neutral lipid was a minor fraction (5% to 10%) of the extractable lipids and was found to consist of free fatty acids, esters, and coenzyme Q.
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Kaneshiro et al. (1962) studied this question.
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