Two atomic constraints unambiguously position the S4 segment relative to S1 and S2 segments in the closed state of Shaker K channel
Population
Shaker K channel
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Provides atomic constraints on K-channel gating; leaves open translation to human cardiac electrophysiology.
Structured PICO
P
Population
Shaker K channel
I
Intervention
Histidine scanning mutagenesis (I241H, I287H) and substituted cysteine residues (I241C, I287C, R362C)
O
Outcome
Position and movement of the S4 segment relative to S1 and S2 segments in the closed statesurrogate
The study provides atomic constraints for the closed-state position of the S4 segment in the Shaker K channel, revealing a ~180-degree axial rotation and ~6.5 A vertical movement during gating.
Cite This Study
Campos et al. (2007) studied this question.