Martin R. McAinsh, Colin Brownlee, Alistair M. Hetherington, Visualizing Changes in Cytosolic-Free Ca2+ during the Response of Stomatal Guard Cells to Abscisic Acid, The Plant Cell, Vol. 4, No. 9 (Sep., 1992), pp. 1113-1122
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McAinsh et al. (1992) studied this question.