The LLC-MK2 cell line is a viable alternative to primary rhesus monkey kidney cultures for isolating Coxsackie B and polioviruses, though less effective for ECHO viruses.
May guide cell line selection for Coxsackie B and polioviruses; leaves open utility for ECHO viruses.
The relative susceptibility of the LLC-MK 2 cell line and primary rhesus monkey kidney cultures to thirty-eight prototype strains of human enteroviruses is described; of these strains only ECHO types 16, 18, 21 and 23 failed to cause CPE in the continuous cell line. The efficiency of the two tissues for the isolation of enteroviruses from faecal extracts is compared. The results show that the LLC-MK 2 cell line is very satisfactory for the isolation of Coxsackie B and polioviruses, but not so useful for the isolation of ECHO viruses. The identification of enteroviruses by neutralization tests in LLC-MK 2 cells is successful.
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Hambling et al. (1965) studied this question.
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