What question did this study set out to answer?

To evaluate the role of 1,25-dihydroxyvitamin D3 in ovarian follicular development and steroidogenesis using a human granulosa cell model.

March 14, 2014The Journal of Clinical Endocrinology & MetabolismOpen Access

Vitamin D Alters Genes Involved in Follicular Development and Steroidogenesis in Human Cumulus Granulosa Cells

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Authors

ZMZaher MerhiUniversity of Vermont ADAngela DoswellUniversity of Vermont KKKendall KrebsUniversity of Vermont

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Implication

In vitro study demonstrates 1,25-dihydroxyvitamin D3 alters AMH signaling and steroidogenesis in human cumulus granulosa cells, suggesting enhanced follicular luteinization.

Key Points

To evaluate the role of 1,25-dihydroxyvitamin D3 in ovarian follicular development and steroidogenesis using a human granulosa cell model.
Enrolled 54 women undergoing in vitro fertilization and collected follicular fluid as well as mural and cumulus granulosa cells from small and large follicles.
Cultured primary cumulus granulosa cells with or without 1,25-dihydroxyvitamin D3 to measure mRNA expression, steroid hormone output (progesterone and estradiol), and recombinant AMH-induced phospho-Smad 1/5/8 nuclear localization.
Follicular fluid 25-hydroxyvitamin D negatively correlated with AMH and AMHR-II mRNA levels, with deficient/insufficient women showing a 2-fold increase in AMHR-II mRNA in small follicle cumulus cells compared to replete controls (P = .02).
Treatment with 1,25-dihydroxyvitamin D3 significantly decreased AMHR-II and FSH receptor mRNA while increasing 3β-HSD mRNA expression and progesterone secretion (P < .05).
1,25-dihydroxyvitamin D3 decreased Smad 1/5/8 phosphorylation and nuclear localization, but it did not alter FSH-induced aromatase mRNA expression or estradiol production.

Cite This Study

Merhi et al. (2014) studied this question.

synapsesocial.com/papers/6a715cabb27f15817827ba4f https://doi.org/10.1210/jc.2013-4161

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Implication

In vitro study demonstrates 1,25-dihydroxyvitamin D3 alters AMH signaling and steroidogenesis in human cumulus granulosa cells, suggesting enhanced follicular luteinization.

Vitamin D Alters Genes Involved in Follicular Development and Steroidogenesis in Human Cumulus Granulosa Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Vitamin D Alters Genes Involved in Follicular Development and Steroidogenesis in Human Cumulus Granulosa Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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