Radioactivityincorporated into an e-N-5'-phosphopyridoxyllysine residue of the /3 chain by reduction of the flZ holoenzyme with tritiated sodium borohydride appears in two tryptic peptides.These pyridoxyl peptides differ by only a single arginyl residue.As expected, tryptic digests of normal protein contain two peptides that together make up the pyridoxyl peptide region.The primary structure in this region was determined to be: Arg-Glu-Asp-Leu-Leu-His-Gly-Gly-Ala-His
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Fluri et al. (1971) studied this question.