Castrated male rats injected daily with 2 micrograms of estradiol benzoate (EB) combined with 200 micrograms of dihydrotestosterone propionate (DHTP) displayed masculine mating behavior which was indistinguishable from that of other castrates treated with 200 micrograms of testosterone propionate (TP). Significantly less copulation was seen in rats treated with either 4 micrograms of TP plus 200 micrograms of DHTP or 2 micrograms of EB. Mating in male rats may depend on the action of both estrogenic and 5alpha-dihydro metabolites of testosterone.
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Baum et al. (1973) studied this question.
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