What question did this study set out to answer?

To determine how patients lacking functional transporter associated with antigen processing (TAP) proteins preserve antiviral immunity despite severely reduced surface HLA class I expression.

May 1, 1997The Journal of Immunology

Human peptide transporter deficiency: importance of HLA-B in the presentation of TAP-independent EBV antigens

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Authors

HSHenri de la SalleUniversité Paris-Sud EHElisabeth HoussaintRoswell Park Comprehensive Cancer Center MPMarie‐Alix PeyratRoswell Park Comprehensive Cancer Center

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Implication

Immunological analysis reveals HLA-B presentation of Epstein-Barr virus antigens in TAP-deficient siblings, indicating functional CD8+ T cell antiviral immunity via TAP-independent pathways.

Key Points

To determine how patients lacking functional transporter associated with antigen processing (TAP) proteins preserve antiviral immunity despite severely reduced surface HLA class I expression.
Characterized cell surface HLA class I expression and sequenced eluted peptides from TAP-deficient Epstein-Barr virus (EBV) B lymphoblastoid cell lines via cytofluorometry and biochemical analysis.
Assessed peripheral alphabeta CD8+ T cell receptor repertoire diversity and isolated cytotoxic CD8+ clones to measure target cell lysis against EBV latent membrane protein 2 (LMP2).
Surface-expressed class I molecules on TAP-deficient cells were predominantly HLA-B products carrying peptides derived from cytosolic proteins.
Isolated an HLA-B-restricted cytotoxic CD8+ alphabeta T cell clone specific for the EBV LMP2 antigen presented on TAP-deficient cells, while 21 of 22 CD8+ T cell clones displayed no reactivity toward normal cells expressing identical HLA alleles.

Cite This Study

Salle et al. (1997) studied this question.

synapsesocial.com/papers/6a7162f3ce524a4339c4eef2 https://doi.org/10.4049/jimmunol.158.10.4555

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Implication

Immunological analysis reveals HLA-B presentation of Epstein-Barr virus antigens in TAP-deficient siblings, indicating functional CD8+ T cell antiviral immunity via TAP-independent pathways.

Human peptide transporter deficiency: importance of HLA-B in the presentation of TAP-independent EBV antigens

Discussion

Member takes

Implication

Key Points

Cite This Study

Human peptide transporter deficiency: importance of HLA-B in the presentation of TAP-independent EBV antigens

Discussion

Member takes

Implication

Key Points

Cite This Study

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