Intracellular K + in lysed freeze-thawed cell pastes of the extreme halophile, H. cutirubrum, diffused readily against a concentration gradient during equilibrium dialysis, whereas the intracellular Mg 2+ was not readily diffusible. The K + activity in the lysed cell pastes, determined directly by K + ion-specific electrode, was comparable to the total K-content of the cells, as determined by atomic absorption. This finding and the results of the dialysis experiment suggest that the intracellular K + in H. cutirubrum is not bound.
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Lanyi et al. (1972) studied this question.