Validation of predictive equations to estimate resting metabolic rate of females and males across different activity levels

Objectives Using equations to predict resting metabolic rate (RMR) has yielded different degrees of validity, particularly when sex and different physical activity levels were considered. Therefore, the purpose of the present study was to determine the validity of several different predictive equations to estimate RMR in female and male adults with varying physical activity levels. Method We measured the RMR of 50 adults (26 females and 24 males) evenly distributed through activity levels varying from sedentary to ultra‐endurance. Body composition was measured by dual X–ray absorptiometry and physical activity was monitored by accelerometry. Ten equations to predict RMR were applied (using Body Mass [BM]: Harris & Benedict, 1919; Mifflin et al., 1990 [Mifflin BM ]; Pontzer et al., 2021 [Pontzer BM ]; Schofield, 1985; FAO/WHO/UNU, 2004; and using Fat‐Free Mass (FFM): Cunningham, 1991; Johnstone et al., 2006; Mifflin et al., 1990 [Mifflin FFM ]; Nelson et al. 1992; Pontzer et al., 2021 [Pontzer FFM ]). The accuracy of these equations was analyzed, and the effect of sex and physical activity was evaluated using different accuracy metrics. Results Equations using BM were less accurate for females, and their accuracy was influenced by physical activity and body composition. FFM equations were slightly less accurate for males but there was no obvious effect of physical activity or other sample parameters. Pontzer FFM provides higher accuracy than other models independent of the magnitude of RMR, sex, activity levels, and sample characteristics. Conclusion Equations using FFM were more accurate than BM equations in our sample. Future studies are needed to test the accuracy of RMR prediction equations in diverse samples.