Why the study?
Does nitric oxide mediate the cooling-induced changes in relaxation of the human umbilical artery?
Does nitric oxide mediate the cooling-induced changes in relaxation of the human umbilical artery?
Cooling-induced changes in the relaxation of the human umbilical artery by diazoxide, isoproterenol, and magnesium sulphate are independent of nitric oxide.
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Cooling impairs relaxation independently of NO in animal models; leaves open relevance to human umbilical artery and requires confirmation.
In the present study, the effects of cooling (to 28°C) on the vasodilatation induced by diazoxide (10(-9)-3×10(-4) M), isoproterenol (10(-9)-3×10(-4) M) and magnesium sulphate (0.1-30 mM) on serotonin-pre-contracted human umbilical artery and the role of nitric oxide in these effects were analyzed. Diazoxide, isoproterenol and magnesium produced concentration-dependent relaxation of human umbilical artery precontracted with serotonin (10(-6) M). During cooling, the pIC(50) values and maximal responses to these agents were significantly lower than at 37°C. Cooling to 28°C in the presence of N(G)-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) did not modify the effects of temperature on diazoxide, isoproterenol and magnesium-induced relaxations. These results suggest that cooling-induced changes of diazoxide, isoproterenol, and magnesium sulphate in human umbilical artery are independent of nitric oxide.
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Atalık et al. (2011) studied this question.
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