Callus formation and shoot regeneration invanilla planifolia

The high demand for natural vanilla flavor led to attempts to establish an in vitro cell culture of Vanilla planifolia for flavor production and for shoot regeneration from callus. Explants from Vanilla planifolia shoots were placed on supplement Murashige and Skoog salts and tested for callus formation. Callus formation could be achieved with various media. Shoots could be regenerated from callus under various concentrations of a‐naphthaleneacetic acid (NAA) and benzyladenine (BA). The first node was the best location for callus initiation with subsequent callus proliferation.